His-tag proteins can be non-covalently immobilized to NTA biosensor chips, and the FLEX biosensor chips immobilize the target using hydrophobic interactions, noncovalent binding, or covalent binding, depending on the target and linking chemistry. These various binding chemistries make Agile flexible and adaptable to established research and development pipelines.
Q: What reagents are supplied by Nanomed?
A: The necessary reagents specific to each biosensor chip are provided with your biosensor chip order.
Q: What reagents do I need to supply to run measurements with Agile?
A: For all biosensor chips, you will need the buffers specific to your target and analyte.
Q: How do I learn to use Agile?
A: The Agile Getting Started Kit (GSK) is a self-training package. The GSK walks you through a positive control interaction (), teaching you how to use the system and understand the analysis provided by Agile Plus software. Additional GSKs can be ordered separately if needed. Agile also comes with an extensive User Manual for reference, and Field Application Scientists are on hand to provide special training to any number of users upon request.
Q: What kind of experimental design or assay optimization help is offered by Nanomed?
A: Field Applications Scientists are on-hand to provide technical support or travel to the site to help with chemistry optimization or other questions related to Agile. Contact technical support to inquire!
Q: How do I immobilize my target to the Agile biosensor chip?
A: There are 5 types of biosensor chips to covalently or non-covalently link your target to the graphene surface: COOH, NHS, NH2, NTA, and FLEX. The COOH biosensor chips come with exposed carboxyl groups to covalently link target directly to the graphene surface using carbodiimide crosslinker chemistry (i.e. EDC/sulfo-NHS). EDC and sNHS activate the carboxyl groups on the surface of the graphene to react with any free amine group in your target and form a covalent amide bond. Alternatively, NHS biosensor chips negate the need for EDC/sNHS while forming a covalent amide bond to a free amine group in your target. The NH2 biosensor chips have amine groups functionalized to the graphene surface, and EDC/sNHS activate the carboxyl groups on the target to react with the amine groups on the biosensor chip surface, forming a covalent amide bond.
Q: How many Agile biosensor chips will I need for my experiment?
A: A single experiment (such as for a dose response curve of a biological interaction) will require 1 biosensor chip or more, and the number of biosensor chips is dependent on the biological interaction of interest. If the interaction between the immobilized target and analyte in solution fully dissociates (with or without regeneration techniques), up to 10 interactions can be measured using a single Agile biosensor chip. If the interaction does not fully dissociate, 1 biosensor chip can measure a single interaction, and the number of biosensor chips needed will equal the number of interactions tested.
Q: How many times can I use an Agile biosensor chip?
A: The Agile biosensor chip can be used for up to 10 measurements, depending on the stability of the immobilized target, stability of the analyte during the experiment, and success of analyte dissociation from the immobilized target.
Q: When I choose transistors in the Agile Plus software, is the data still being recorded?
A: Yes, when you choose transistors in the Agile Plus software, the data from every transistor continues to record. Only the data for the selected transistors is displayed, but all data is recorded.
Q: What is the High Density Cable Accessory and how do I use it?
A: The Agile cartridge connects directly to the reader for compact use. The cartridge can also attach to the reader via a specially-designed one-meter-long high density cable, which is an optional accessory. This enables additional use of the cartridge in a wide range of experimental settings (ex: with a temperature-controlled incubator or in a biosafety cabinet) that may otherwise damage the reader. The cartridge and VHD cable can be separated from the reader post-experiment for easy decontamination with standard protocols.
Q: What are the dimensions of the system?
A: The cartridge is approximately (L x W x H) 3.2” x 3” x 1.4”, and the reader is approximately 5.5” x 3” x 1.4”.
Q: What is the power supply of Agile?
A: Agile connects to a computer via a USB to micro-USB cable and is powered by the computer. Agile requires minimal processing power, and a laptop or computer running other applications can power Agile.