Application Note: Reproducible Kinetic Characterization of Small Molecule Compounds in Serum with Agile R100
In this application note, we share buffer and serum interactions for 3 target and small molecule binding pairs: a chemokine binding G-protein coupled receptor (CR GPCR) binding with small molecule Compound A, the cytokine tumor necrosis factor alpha (TNFα) protein binding with small molecule SPD304, and a DNA aptamer against colistin (i.e. colistin aptamer) binding with the antibiotic drug colistin. No assay adjustment is made in the transition from buffer to serum.
All kinetic constants between buffer and serum for the 3 binding interactions are within a factor of 1.9, displaying a high level of precision among multiple replicates and measurements.