Why the New SCOOH Biosensor Is Great For High Concentration Measurements
False positives are problematic in early drug discovery when libraries are typically screened at high concentrations. Assay accuracy can be affected by multiple mechanisms, including high levels of solvent used to keep small molecules in aqueous solution, and nonspecific hydrophobic binding.
We’ve designed our new SCOOH Agile biosensor to meet these challenges. As an electrical sensing platform, Agile R100 is unaffected by optical impediments like solvents and detergents, enabling dependable results in up to 10% DMSO. The newly-launched SCOOH biosensor ups the ante with a layer of hydrogel covalently bonded to the sensor surface that drastically reduces nonspecific binding. The hydrogel layer is comprised of polymer brushes loaded with 25 carboxyl groups each, which create a dense hydrophilic surface that prevents nonspecific noise.
As a bonus, the carboxyl polymer brushes provide the SCOOH biosensor with 25 times the number of binding sites of the COOH biosensor. The increased binding capacity increases the likelihood of accurate results when measuring low affinity interactions, making SCOOH an ideal surface chemistry to measure weak binders.
The new SCOOH Agile Biosensor.